μBS Lab

Laboratory of Experimental Micro and Biofluid dynamics

mimiclab

The mission of the laboratory is to carry out research and teaching in the fields of in-vitro biofluid-dynamics and development of devices interacting with biological fluids and tissues. In line with these activities, µBS Lab provides the Biomech group with multipurpose fluid-dynamic bench testing capabilities, representing the main support environment for the assessment of our thematic laboratories’ technological developments.
Research at µBS Lab is carried out in close contact with laboratories of excellence at other PoliMI Departments and with National and International research centers. Continuous collaborations with Italian and International biomedical industries also take place, particularly in the fields of blood processing apparatuses and devices for cardiovascular surgical/interventional corrections.
µBS Lab supports the activities of the FoRCardioLab, the laboratory of the Foundation for Research in Cardiac Surgery, located at L. Sacco hospital in Milan, a joint initiative of PoliMI researchers in biomedical engineering and biomedical staff at the University of Milan.
Lab activities of the Biofluidodynamics Laboratory teaching course takes place entirely at µBS Lab, as well as other lab activities pertaining to some other teaching courses of the Master's Degree Program in Biomedical Engineering.

 

 

Research interests

The aim of the research at µBS Lab is improving knowledge in the fields of cardiovascular bioengineering, biological fluids treatment, tissue engineering and regenerative medicine. The aims of basic research are then combined with those of applied research, mainly in terms of development and optimization of biomedical devices for lab research and/or for industrial applications.
Specifically, research is devoted the following methodological and technological aspects:

a. Experimental studies of cardiovascular fluid dynamics in physiological, pathological and/or prosthesized conditions

b. Functional analysis and optimization of devices for the treatment of blood (filters, mass and heat exchangers, dialysers, etc.)

c. Design, realization and testing of tissue culture systems for advanced cardiovascular mechanobiology studies and for the regenerative medicine field

d. Complementary topics of biomedical fluid dynamics, such as the study of cerebrospinal fluid treatments and advanced techniques for eye surgery

 

Team

Faculties  

Gianfranco Beniamino Fiore, PhD

Associate Professor
Experimental Micro- and Bio- Fluid Dynamics

Tel.: +39-02-2399-3337
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Monica Soncini, PhD

Associate Professor
Tissue Engineering

Tel.: +39-02-2399-3336
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Riccardo Vismara, PhD

Assistant Professor
Experimental Bio-Fluid Dynamics

Tel.: +39-02-2399-4142
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Research Fellows 

Michal Jaworek, MSc

PhD Candidate, Research Fellow
Experimental Bio-Fluid Dynamics

Tel.: +39-02-2399-3327
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Federico Lucherini, MSc

Research Fellow
3D printing, Experimental Bio-Fluid Dynamics

Tel.: +39-02-2399-3327
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PhD Students  

Simona Seminati, MSc

PhD Candidate
Cell and Tissue Bioengineering

Tel.: +39-02-2399-3370
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Lorenzo Coppadoro, MSc

PhD Candidate
Cell and Tissue Bioengineering

Tel.: +39-02-2399-3370
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Master Students  

  Claudia Vecchiato, BSc

  Elia Pederzani, BSc

  Francesca Perico, BSc

  Giulia Rottoli, BSc

Alumni

2018

Lorenzo Pietro Coppadoro, Msc

Beatrice Rossi, Msc

Giulia Parigi, Msc

Lorenzo Pecoraro, Msc

Cecilia Brambilla Pisoni, Msc

2017

Michal Bugala, Msc

Valentina Caccetta, Msc

2016

Simona Seminati, Msc

Giorgia Milotta, Msc

2014

Martina Malavasi, Msc

Michela Pin, Msc

Alessandra Marzorati, Msc

Marco Spiccia, MSc

2013

Emilio Savoldelli, Msc

Elena Bresciani, Msc

Alice Cambiaghi, PhD

Francesco Dal Dosso, MSc

2012

Lorena Casadonte, Msc

Nina Bono, PhD

Alberto Urban, MSc

Claudia Samà, MSc

 

  

Selected Publications

 

Selected Publications

  1. Strobel HA, Hookway TA, Piola M, Fiore GB, Soncini M, Alsberg E, Rolle MW. Assembly of Tissue-Engineered Blood Vessels with Spatially Controlled Heterogeneities. Tissue Eng Part A. 2018 Oct;24(19-20):1492-1503. doi: 10.1089/ten.TEA.2017.0492.

  2. Suchý T, Šupová M, Bartoš M, Sedláček R, Piola M, Soncini M, Fiore GB, Sauerová P, Kalbáčová MH. Dry versus hydrated collagen scaffolds: are dry states representative of hydrated states? J Mater Sci Mater Med. 2018 Feb 1;29(3):20. doi: 10.1007/s10856-017-6024-2.

  3. Bono N, Meghezi S, Soncini M, Piola M, Mantovani D, Fiore GB. A Dual-Mode Bioreactor System for Tissue Engineered Vascular Models. Ann Biomed Eng. 2017 Jun;45(6):1496-1510. doi: 10.1007/s10439-017-1813-9.

  4. Piola M, Ruiter M, Vismara R, Mastrullo V, Agrifoglio M, Zanobini M, Pesce M, Soncini M, Fiore GB. Full Mimicking of Coronary Hemodynamics for Ex-Vivo Stimulation of Human Saphenous Veins. Ann Biomed Eng. 2017 Apr;45(4):884-897. doi: 10.1007/s10439-016-1747-7.

  5. Ugolini, G. S.; Pavesi, A.; Rasponi, M.; Fiore, G. B.; Kamm, R.; Soncini, M. Human cardiac fibroblasts adaptive responses to controlled combined mechanical strain and oxygen changes in vitro. Elife 2017 Mar; 6.pii: e22847. doi: 10.7554/eLife.22847
  6. Ugolini, G. S.; Rasponi, M.; Pavesi, A.; Santoro, R.; Kamm, R.; Fiore, G. B.; Pesce, M.; Soncini, M. On-chip assessment of human primary cardiac fibroblasts proliferative responses to uniaxial cyclic mechanical strain. Biotechnol Bioeng. 2016 Apr;113(4):859-69. doi: 10.1002/bit.25847.
  7. Piola M, Prandi F, Fiore GB, Agrifoglio M, Polvani G, Pesce M, Soncini M. Human Saphenous Vein Response to Trans-wall Oxygen Gradients in a Novel Ex Vivo Conditioning Platform. Ann Biomed Eng. 2016 May;44(5):1449-61. doi: 10.1007/s10439-015-1434-0. Epub 2015 Aug 29. Erratum in: Ann Biomed Eng. 2015 Dec;43(12):3040.

  8. Piola M, Prandi F, Bono N, Soncini M, Penza E, Agrifoglio M, Polvani G, Pesce M, Fiore GB. A compact and automated ex vivo vessel culture system for the pulsatile pressure conditioning of human saphenous veins. J Tissue Eng Regen Med. 2016 Mar;10(3):E204-15. doi: 10.1002/term.1798.

  9. Santoro R, Consolo F, Spiccia M, Piola M, Kassem S, Prandi F, Vinci MC, Forti E, Polvani G, Fiore GB, Soncini M, Pesce M. Feasibility of pig and human-derived aortic valve interstitial cells seeding on fixative-free decellularized animal pericardium. J Biomed Mater Res B Appl Biomater. 2016 Feb;104(2):345-56. doi: 10.1002/jbm.b.33404. 

  10. Consolo F, Brizzola S, Tremolada G, Grieco V, Riva F, Acocella F, Fiore GB, Soncini M. A dynamic distention protocol for whole-organ bladder decellularization: histological and biomechanical characterization of the acellular matrix. J Tissue Eng Regen Med. 2016 Feb;10(2):E101-12. doi: 10.1002/term.1767.

  11. Piola M, Ruiter M, Vismara R, Mastrullo V, Agrifoglio M, Zanobini M, Pesce M, Soncini M, Fiore GB. Full Mimicking of Coronary Hemodynamics for Ex-Vivo Stimulation of Human Saphenous Veins. Ann Biomed Eng. 2016 Oct 17.

  12. Bono N, Pezzoli D, Levesque L, Loy C, Candiani G, Fiore GB, Mantovani D. Unraveling the role of mechanical stimulation on smooth muscle cells: A comparative study between 2D and 3D models. Biotechnol Bioeng. 2016 Oct;113(10):2254-63. doi: 10.1002/bit.25979.

  13. Prandi F*, Piola M*, Soncini M, Colussi C, D'Alessandra Y, Penza E, Agrifoglio M, Vinci MC, Polvani G, Gaetano C, Fiore GB, Pesce M. CAdventitial vessel growth and progenitor cells activation in an ex vivo culture system mimicking human saphenous vein wall strain after coronary artery bypass grafting. PLoS One. 2015 Feb 17;10(2):e0117409. doi: 10.1371/journal.pone.0117409. eCollection 2015.

  14. Consolo F, Fiore GB, Pelosi A, Reggiani S, Redaelli A. A numerical performance assessment of a commercial cardiopulmonary by-pass blood heat exchanger. Med Eng Phys. 2015 Jun;37(6):584-92. DOI: 10.1016/j.medengphy.2015.03.002.

  15. Santoro R, Consolo F, Spiccia M, Piola M, Kassem S, Prandi F, Vinci MC, Forti E, Polvani G, Fiore GB, Soncini M, Pesce M. Feasibility of pig and human-derived aortic valve interstitial cells seeding on fixative-free decellularized animal pericardium. J Biomed Mater Res B Appl Biomater. 2015 Mar 23. DOI: 10.1002/jbm.b.33404.

  16. Pietronave S, Zamperone A, Oltolina F, Colangelo D, Follenzi A, Novelli E, Diena M, Pavesi A, Consolo F, Fiore GB, Soncini M, Prat M. Monophasic and biphasic electrical stimulation induces a precardiac differentiation in progenitor cells isolated from human heart. Stem Cells Dev. 2014 Apr 15;23(8):888-98.

  17. Piola M, Soncini M, Cantini M, Sadr N, Ferrario G, Fiore GB. Design and functional testing of a multichamber perfusion platform for three-dimensional scaffolds. ScientificWorldJournal. 2013 Dec 23;2013:123974. doi: 10.1155/2013/123974. eCollection 2013.

  18. Apicella A, Soncini M, Deriu MA, Natalello A, Bonanomi M, Dellasega D, Tortora P, Regonesi ME, Casari CS. A hydrophobic gold surface triggers misfolding and aggregation of the amyloidogenic Josephin domain in monomeric form, while leaving the oligomers unaffected. PLoS One. 2013;8(3):e58794.

  19. Vinci MC, Tessitore G, Castiglioni L, Prandi F, Soncini M, Santoro R, Consolo F, Colazzo F, Micheli B, Sironi L, Polvani G, Pesce M. Mechanical compliance and immunological compatibility of fixative-free decellularized/cryopreserved human pericardium. PLoS One. 2013 May 21;8(5):e64769.

  20. Biffi E, Regalia G, Ghezzi D, De Ceglia R, Menegon A, Ferrigno G, Fiore GB, Pedrocchi A. A novel environmental chamber for neuronal network multisite recordings. Biotechnol Bioeng 2012 Oct;109(10):2553-66. doi: 10.1002/bit.24526.

  21. Forvi E, Bedoni M, Carabalona R, Soncini M, Mazzoleni P, Rizzo F, O'Mahony C, Morasso C, Cassarà DG, Gramatica F. Preliminary technological assessment of microneedles-based dry electrodes for biopotential monitoring in clinical examinations. Sensor Actuat A Phys. 2012; 180:177-186.

  22. Piola M, Soncini M, Prandi F, Polvani G, Fiore GB, Pesce M. Tools and Procedures for Ex Vivo Vein Arterialization, Preconditioning and Tissue Engineering: A Step Forward to Translation to Combat the Consequences of Vascular Graft Remodeling. Recent Pat Cardiovasc Drug Discov. 2012; 7(3):186-195.

  

Prizes and Honors

 

... coming soon!

 

 

Collaborations

 

Collaborations

 

... coming soon!

 

Equipment and available technologies

 

Equipment

 

... coming soon!

 

 

Funding

 

 

... coming soon!

 

Open positions

........ coming soon!

 

Contacts and Location

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Building 21 – Via Golgi 39 – Phone: +39-02-2399-3337

 
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