Th-Lab

Laboratory for the analaysis of Thrombogenicity

mimiclab


Th-Lab is dedicated to the analysis of the thrombogenic potential of blood medical devices with specific emphasis on shear-mediated platelet activation. The research activities aim at developing point-of-care systems for the assessment of platelet function at the bed-side, in order to prevent thromboembolic complications through an effective and efficient drug management.

 

 

 

 

Research interests

Current projects focus on the following basic and applied research activities:

Thrombogenicity of blood contacting medical devices

  • Microfluidic flow-based platforms, able to stimulate platelets with abnormal shear-stress patterns typical of blood medical devices (mechanical heart valves, ventricular assist devices and blood oxygenators)
  • Microfluidic flow-based platforms for testing the efficacy of antiplatelet drugs under hyper-shear conditions
  • Device Thrombogenicity Emulator (DTE): combining advanced computational and experimental techniques for optimizing the thrombogenic performance of cardiovascular devices
  • Evaluation of the thrombogenicity of material surfaces of blood-contacting devices

Platelet function testing

  • Point-of-care platelet aggregation systems, integrating microfluidics, electronics and impedance measurements
  • Shear-mediated thrombus formation on collagen-coated microchannels, mimicking physiologic and stenotic arteries

In vitro thrombogenicity testing

  • Platelet activity state (PAS) assay, allowing the measurement of the real-time thrombin production rate of activated platelets
  • Hemodynamic Shearing Device (HSD) a state-of-the-art benchtop system designed to expose platelets to defined levels and patterns of shear-stress

Platelet-on-chip technologies

  • Lab-on-a chip systems for continuous platelet separation from whole blood
  • On-chip platelet cell counter for rapid and automated measurement of platelet concentration

Team

Faculties  

Alberto Redaelli, PhD

Full Professor
Computational Fluid Dynamics

Tel.: +39-02-2399-3375
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Giuseppe Passoni, PhD

Associate Professor
Computational Fluid Dynamics

Tel.: +39-02-2399-6232
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Silvia Bozzi, PhD

Associate Professor
Computational Fluid Dynamics

Tel.: +39-02-2399-4146
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Master Students  

  Agnese Manzoni, BSc

  Andrea Mazzoleni, BSc

  Chiara Ferrari, BSc

  Dario Biancospino, BSc

  Elisabetta Russo, BSc

  Federica Vercellino, BSc

  Gabriele Mantica, BSc

  Marta Bonora, BSc

  Stefano Andrea Rizzo, BSc

  Tatiana Mencarini, BSc

  Tomaso Gianiorio, BSc 

Alumni

Filippo Consolo, PhD

Annalisa Dimasi, PhD

Matteo Nobili, PhD

Andrea Santoleri, PhD

Giulia Sferrazza, MSc

Giulia Motolone, MSc

Gabriella Sarno, MSc

Silvia Sammartino, MSc

Stefano Speranza, MSc

Roberta Testa, MSc

Maria Grazia Pietropaolo, MSc

Giulia Marazzato, MSc

Valentina Vincoli, MSc

 

 

  

Publications

 

Selected Publications

  1. Apostoli A,  Bianchi V,  Bono N,  Dimasi A,  Ammann KR,  Moiia YR,  Montisci A,  Sheriff J,  Bluestein D,  Fiore GB,  Pappalardo F,  Candiani G,  Redaelli A,  Slepian MJ,  Consolo F. Prothrombotic activity of cytokine-activated endothelial cells and shear-activated platelets in the setting of ventricular assist device support. The Journal of Heart and Lung Transplantation 2019, doi: 10.1016/j.healun.2019.02.009.

  2. Selmi M, Chiu WC, Chivukula VK, Melisurgo G, Beckman JA, Mahr C, Aliseda A, Votta E, Redaelli A, Slepian MJ, Bluestein D, Pappalardo F, Consolo F. Blood damage in Left Ventricular Assist Devices: Pump thrombosis or system thrombosis? International Journal of Artificial Organs 2018, Oct 24:391398818806162, doi: 10.1177/0391398818806162.

  3. Consolo F, Sferrazza G, Motolone G, Pieri M, De Bonis M, Zangrillo A, Redaelli A, Slepian MJ, Pappalardo F. Shear-mediated platelet activation enhances thrombotic complications in patients with LVAD and is reversed after heart transplantation. ASAIO Journal 2018, Jun 25, doi: 10.1097/MAT.0000000000000842.

  4. Consolo F,  Sferrazza G,  Motolone G,  Contri R,  Valerio L,  Lembo R,  Pozzi L,  Della Valle P,  De Bonis M,  Zangrillo A,  Fiore GB,  Redaelli A,  Slepian MJ,  Pappalardo F.  Platelet activation is a preoperative risk factor for the development of thromboembolic complications in patients with continuous-flow left ventricular assist device. European Journal of Heart Failure 2018, 20:792-800.

  5. Dimasi A, Roka-Moiia Y, Consolo F, Rasponi M, Fiore GB, Slepian MJ, Redaelli A. Microfluidic flow-based platforms for induction and analysis of dynamic shear-mediated platelet activation-Initial validation versus the standardized hemodynamic shearing device. Biomicrofluidics 2018, 12:042208.

  6. Consolo F, Sheriff J, Gorla S, Magri N, Bluestein D, Pappalardo F, Slepian MJ, Fiore GB, Redaelli A. High frequency components of hemodynamic shear stress profiles are a major determinant of shear-mediated platelet activation in therapeutic blood recirculating devices. Scientific Reports 2017, 7:4994.

  7. Dimasi A, Rasponi M, Consolo F, Fiore GB, Bluestein D, Slepian MJ, Redaelli A. Microfluidic platforms for the evaluation of anti-platelet agent efficacy under hyper-shear conditions associated with ventricular assist devices. Medical Engineering and Physics 2017, 48:31-38.

  8. Consolo F, Dimasi A, Rasponi M, Valerio L, Pappalardo F, Bluestein D, Slepian MJ, Fiore GB, Redaelli A. Microfluidic approaches for the assessment of blood cell trauma: a focus on thrombotic risk in mechanical circulatory support devices. International Journal of Artificial Organs 2016, 39:184-193.

  9. Consolo F, Valerio L, Brizzola S, Rota P, Marazzato G, Vincoli V, Reggiani S, Redaelli A, Fiore G. On the use of the platelet activity state assay for the in vitro quantification of platelet activation in blood recirculating devices for extracorporeal circulation. Artificial Organs 2016, 40(10):971-980.

  10. Dimasi A, Rasponi M, Sheriff J, Chiu WC, Bluestein D, Tran PL, Slepian MJ, Redaelli A. Microfluidic emulation of mechanical circulatory support device shear-mediated platelet activation. Biomedical Microdevices 2015, 17:117.

      

Prizes and Honors

 

........ coming soon! 

 

 

Collaborations

 

Collaborations


 

University of Arizona
Sarver Heart Center

Collaborations on: Point-of-care devices for platelet function testing

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Stony Brook University
Biofluids Research Group

Collaborations on: Thrombogenicity of blood recirculating devices

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Pennsylvania State University
Artificial Heart and Cardiovascular Fluid Dynamics Lab

Collaborations on: In vitro and in silico modelling of device-induced thrombosis

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Nanyang Technological University
MicroBioengineering and microfluidics Research Group

Collaborations on: Microfluidic cell-sorting devices

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Università Vita-Salute San Raffaele
Anesthesia and Cardiothoracic Intensive Care

Collaborations on: In vitro thrombogenicity testing

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Equipment and available technologies

 

Equipment and Available Technologies

Laboratory facilities include equipment and reagents for gel-filtered platelet separation and prothrombinase assay studies:

  • laboratory water-bath
  • benchtop centrifuge
  • four gel-chromatography columns
  • two peristaltic pumps
  • two syringe pumps
  • Hamilton PSD/8 precision syringe pump
  • pH-meter
  • optical refractometer
  • thermocouple probes
  • Coulter Z2 particle counter
  • microprobe sonicator
  • multi-well microreader spectrophotometer
  • Hemodynamic Shearing Device (HSD) with stepper motor and controller (as part of the Device Thrombogenicity Emulator setup)

 

Funding

 

Th-Lab is grateful to the following funders and partners that have supported our research:

Cariplo Bank Foundation

“Platelet activation in a Lab on Chip device (PlatLoC)” (2016-2017)

“Evaluation of the thrombogenic risk: development of new approaches for the prediction of platelet activation and its minimization in artificial organs and prostheses (FluTE)” (2011-2015)

  

Open positions

........ coming soon!

 

Contacts and Location

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Building 21 – Via Golgi 39 – Phone: +39-02-2399-4146

 
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